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NOAH-( 15 N/ 13 C)-CEST NMR supersequence for dynamics studies of biomolecules

https://doi.org/10.1039/D2CC02015G

Cabrera Allpas, Rodrigo ; Hansen, Alexandar L. ; Brüschweiler, Rafael ( August 2022 , Chemical Communications)

An NMR supersequence is introduced for the rapid acquisition of 15 N-CEST and methyl- 13 C-CEST experiments in the same pulse sequence for applications to proteins. The high sensitivity and accuracy allows the simultaneous quantitative characterization of backbone and side-chain dynamics on the millisecond timescale ideal for routine screening for alternative protein states.
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